Molecular docking, synthesis and anticancer activity of thiosemicarbazone derivatives against MCF-7 human breast cancer cell line

The aim of this study was to synthesize and evaluate anticancer activity 2-hydroxy benzaldehyde 4-hydroxy thiosemicarbazone (2-HBTSc 4-HBTSc) against MCF-7 breast cancer cell line. ligands were prepared characterized by UV vis, IR NMR. MTT assay used assess viability cells. RNA isolation, extraction cDNA synthesis done. Then all groups subjected RT-qPCR using Gene expression specific primers. Also, western blot protein molecular docking Two-way ANOVA with Tukey post-hoc test employed the significance GraphPad Prism. IC 50 values 3.36?g/ml 3.60?g/ml for 2-HBTSc 4-HBTSc treated tumor cells respectively. Tumor growth inhibition ranged from 38 49.27% in cells, 19 25% increase doses 5 ?g/ml 20 ?g/ml. gene result showed a significant upregulation suppressor apoptosis inducing genes while, oncogene significantly downregulated. Specifically, BRCA2 pRB highest Conversely, RAS downregulated significantly. Docking that both have potential inhibit Estrogen Receptor Alpha Ligand Binding Domain, Human 17-Beta-hydroxysteroid dehydrogenase type 1 mutant Topoisomerase II alpha are expressed more during Breast Cancer. findings imply compound has further study. • 2-Hydroxy 4-Hydroxy synthesized characterized. Cytotoxic ligand human line compound. downregulation oncogenes. Molecular enzymes exhibited better activity.